phospho-cytokeratin-8 (K8) (Ser73), Mouse anti-Mouse, Human, Clone: LJ4, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT34425UG-Each-of-1 | In Stock | ₹ 17,118.26 |
MABT34425UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
phospho-cytokeratin-8 (K8) (Ser73)
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
KRT8;CYK8
Immunogen
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity
25 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001243222
Target Species
Mouse, Human
Form
Purified
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Clone
LJ4
Dilution
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Gene Alias
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-phospho-cytokeratin-8 (K8) (Ser73), clone LJ4, Cat
- No
- MABT344, is a mouse monoclonal antibody that detects human type II cytoskeletal 8 phophrorylated on serine 73 and murine cytokeratin-8 phophorylated on serine 79 and has been tested for use in ELISA, Immunocytochemistry, Immunofluoresce Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8 Keratin-8, K8, Type-II keratin Kb80) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human
- Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
- Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73
- Serine 23 is shown to be highly conserved in all type II keratins
- Phosphorylation at Serine 73 is reported to increase during cellular stress, including hear and drug exposure
- However, under normal conditions serine 73 remains largely dephosphorylated
- Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation
- Mutations in KRT8 gene have been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper
- Two isoforms of Cytokeratin-8 have been described that are produced by alternative splicing.