MilliporeSigma™ anti-Prelamin-A Clone: 7G11,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT345MI-Each-of-1 | In Stock | ₹ 42,967.42 |
MABT345MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,967.42
GST (18%): ₹ 7,734.136
Total Price: ₹ 50,701.556
Antigen
Prelamin-A
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
P48678
Host Species
Rat
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 7G11 detects prelamin-A regardless of its farnesylation state, but not mature Lamin-A (a.a. 1-647) lacking the C-terminal propeptide sequence. Clone 7G11 does not detect the pre- or mature forms of the spliced isoforms C1 & C2 (Lamin-C; P48678-2 and P48678-3).
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG2a κ
Applications
Immunofluorescence, Western Blot
Clone
7G11
Formulation
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
Lmna, Lmn1
Immunogen
Linear peptide corresponding to the C-terminal propeptide sequence of mouse Prelamin-A.
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001002011
Target Species
Mouse
Form
Purified
Related Products
Description
- Specifically detects Prelamin-A Clone: 7G11 in Mouse samples, and it is validated for Immunofluorescence, Western Blotting Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size
- The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3)
- Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A that terminals in a CAAX motif
- It is further processed post-translationally by four enzymatic steps to yield the mature Lamin-A
- Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a
- 663-665), carboxylmethylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a
- 648-662), including the farnesylated Cys662, is catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.