Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | OMA103061-Each-of-1 | In Stock | ₹ 50,285.00 |
OMA103061 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,285.00
GST (18%): ₹ 9,051.30
Total Price: ₹ 59,336.30
Antigen
Phospho-AKT1 (Ser473)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
Dulbecco's PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide; pH 7.3
Gene Accession No.
P31749, P31750
Gene Symbols
AKT1
Immunogen
Synthetic phosphopeptide derived from a region of human PKB that contains serine 473.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
Applications
Immunocytochemistry, Western Blot
Clone
14-6
Conjugate
Unconjugated
Gene
AKT1
Gene Alias
Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
11651, 207
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- OMA1-03061 detects protein kinase B (PKB) phosphorylated at Ser473 from human and mouse samples
- OMA1-03061 detects PKB 1,2,and 3
- OMA1-03061 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures
- By Western blot, this antibody detects an ∽55 kDa protein representing phosphorylated PKB (Ser473) from NIH-3T3 cells treated with platelet-derived growth factor (PDGF)
- The OMA1-03061 immunizing phosphopeptide was derived from a region of human PKB that contains serine 473
- There is 100% homology with other species, that have not yet been tested
- Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit
- This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays
- It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications
- Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay
- AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival
- The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells
- This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors
- Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9
- The AKT pathway is a major target for cancer drug discovery.