PA11054
Phospho-IRS1 (Ser307) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PA11054-Each-of-1 | In Stock | ₹ 49,528.50 |
PA11054 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 49,528.50
GST (18%): ₹ 8,915.13
Total Price: ₹ 58,443.63
Antigen
Phospho-IRS1 (Ser307)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
IRS1
Gene Alias
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16367, 3667
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Applications
Immunocytochemistry, Western Blot
Concentration
1 mg/mL
Formulation
PBS with 1mg/mL BSA and 0.05% sodium azide
Gene Accession No.
P35568, P35569
Gene Symbols
IRS1
Immunogen
Synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
Related Products
Description
- PA1-1054 detects phosphorylated IRS-1 (Ser307) in mouse and human cells
- PA1-1054 has been successfully used in Western Blot procedures
- By WB, this antibody detects an ∽170 kDa protein representing phospho-IRS-1 (Ser307) from 3T3 L1 cells treated with insulin for 10 minutes
- The PA1-1054 immunogen is a synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1
- This peptide is 100% conserved in rats and mice
- This peptide (Cat
- #PEP-184) is available for use in neutralization and control experiments
- IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
- IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
- An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
- Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
- Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
- IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.