Phospho-IRS1 (Tyr1179) Polyclonal Antibody, Invitrogen™

Description

• PA1-1060 detects phosphorylated IRS-1 (Tyr1173) in mouse and human cells
• PA1-1060 has been successfully used in Western blot procedures
• By Western blot, this antibody detects a ∽170 kDa protein representing phospho-IRS-1 (Tyr1173) from 3T3 L1 cells treated with insulin
• The PA1-1060 immunogen is a synthetic phosphopeptide corresponding to residues L(1173) E N G L N (pY) I D L D L V K(1186) of human IRS-1
• This immunogenic sequence is 100% conserved in humans and chickens and 85% and 87% conserved in rat and mouse, respectively
• The conserved phosphorylation site corresponds to residue Y1179 in human, Y1177 in chicken, Y1173 in mouse, and Y1172 in rat
• This peptide (Cat
• # PEP-205) is available for use in neutralization and control experiments
• IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
• IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
• An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
• Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
• Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
• IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.