Cyclin E Polyclonal Antibody, Invitrogen™

Description

• PA1-4123 detects Cyclin E1 in human samples
• PA1-4123 has been successfully used in Western blot procedure
• By Western blot this antibody detects a ∽40 kDa protein representing Cyclin E1 in transfected 293T cells
• The PA1-4123 immunogen is a synthetic peptide corresponding to residues G(43) S Q P W D N N A V C(52) of Cyclin E1
• Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle
• Cyclins function as regulators of CDK kinases
• Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event
• Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition
• This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase
• Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis
• This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB
• Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described
• Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.