TRIM28 Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PA1852-Each-of-1 | In Stock | ₹ 42,586.50 |
PA1852 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,586.50
GST (18%): ₹ 7,665.57
Total Price: ₹ 50,252.07
Antigen
TRIM28
Classification
Polyclonal
Conjugate
Unconjugated
Gene
TRIM28
Gene Alias
AA408787; E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; Krip1; KRIP-1; MommeD9; Nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; tif1 beta; Tif1b; Tif1beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; Trim28; tripartite motif; tripartite motif containing 28; tripartite motif protein 28; tripartite motif-containing 28; tripartite motif-containing protein 28
Host Species
Rabbit
Purification Method
Ammonium sulfate precipitation
Regulatory Status
RUO
Gene ID (Entrez)
10155, 116698, 21849
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration
Conc. Not Determined
Formulation
PBS with 0.05% sodium azide
Gene Accession No.
O08629, Q13263, Q62318
Gene Symbols
TRIM28
Immunogen
Recombinant human KAP-1.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- PA1-852 detects KAP-1 (Kruppel-associated box-associated protein 1) in human, mouse and rat samples
- PA1-852 has been successfully used in Western blot, ICC/IF and IHC (P) procedures
- By Western blot, this antibody detects an ∽87 kDa protein representing recombinant human KAP-1
- This antibody also detects a smaller protein that is believed to be KAP-1 degradation product
- In immunofluorescent applications, this antibody primarily reacts with mouse or rat and shows non-specific staining in HeLa cells
- The PA1-852 antigen is a recombinant human KAP-1
- The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors
- There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome
- These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1)
- KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger
- KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression
- KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1)
- KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression
- Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.