PCNA Monoclonal Antibody (PC10), Biotin, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA511355-Each-of-1 | In Stock | ₹ 53,311.00 |
PIMA511355 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,311.00
GST (18%): ₹ 9,595.98
Total Price: ₹ 62,906.98
Antigen
PCNA
Classification
Monoclonal
Concentration
200 μg/mL
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
Gene Accession No.
P04961, P12004
Gene Symbols
Pcna
Immunogen
Recombinant rat PCNA protein
Quantity
500 μL
Primary or Secondary
Primary
Target Species
Canine, Human, Non-human Primate, Rat
Product Type
Antibody
Isotype
IgG2a κ
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone
PC10
Conjugate
Biotin
Gene
Pcna
Gene Alias
ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; MGC8367; Pcna; pcna protein; Pcna/cyclin; PCNAR; POL30; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
25737, 477166, 5111
Content And Storage
4° C
Form
Liquid
Related Products
Description
- Methanol fixation is required for immunofluorescence and flow cytometry staining
- To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended
- By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ∽72kD and 130kD
- PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways
- PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT)
- PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e
- relevant to prognosis)
- PCNA is a marker for cells in early G1 phase and S phase of the cell cycle
- PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication
- In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway
- Two transcript variants encoding the same protein have been found for PCNA
- Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.