ATF5 Recombinant Rabbit Monoclonal Antibody (SD2099), Invitrogen™

Description

• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• ATF5 is a protein coding gene
• Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters
• Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation
• Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4
• Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons
• Must be down-regulated in order for such cells to exit the cycle and differentiate
• Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation
• Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes
• May be involved in osteogenic differentiation
• Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1
• Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1
• Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent
• Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver
• In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase
• May act as a negative regulator of IL1B transduction pathway in liver
• Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members
• Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation
• Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner
• Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.