Kappa Light Chain Monoclonal Antibody (TB28-2), PE-Cyanine7, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 25-997-042-Each-of-1 | In Stock | ₹ 16,732.00 |
25-997-042 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,732.00
GST (18%): ₹ 3,011.76
Total Price: ₹ 19,743.76
Antigen
Kappa Light Chain
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.
P01601, P01602, P01834, P0DOX7
Gene Symbols
IGK, IGKC, IGKV1-5, IGKV1D-16
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
28299, 28901, 3514, 50802
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
TB28-2
Conjugate
PE-Cyanine7
Gene
IGK
Gene Alias
D4Mit8, HCAK1, ig kappa chain c region, Igk, Igk@, Igkc, Igk-C, IGKCD, IGKV, IGKV15, IGKV1-5, Immunoglobulin K, immunoglobulin kappa chain complex, immunoglobulin kappa constant, immunoglobulin kappa light chain, immunoglobulin kappa locus, immunoglobulin kappa variable 1-5, kappa, Kappa LC, Km, L12, L12a, LOC500183, similar to NGF-binding Ig light chain, V1
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin
- Approximately 22-kDa, the Ig kappa chain is comprised of one variable and one constant region
- Two kappa chains together with the heavy chain comprise the whole Ig structure
- Expression of Ig kappa is typically found on more than half of B lymphocytes
- Applications Reported: This TB28-2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This TB28-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product No
- 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
- 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa)
- Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region
- The ratio of kappa to lambda is 70:30
- Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas
- Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.