50-033-382
SIGLEC H Monoclonal Antibody (eBio440c), eFluor™ 660, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-033-382-Each-of-1 | In Stock | ₹ 31,506.00 |
50-033-382 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,506.00
GST (18%): ₹ 5,671.08
Total Price: ₹ 37,177.08
Antigen
SIGLEC H
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
0
Gene Symbols
Siglech
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
233274
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
eBio440c
Conjugate
eFluor 660
Gene
Siglech
Gene Alias
6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2b κ
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Description
- Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6
- Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.