Phospho-Mdm2 (Ser185)Rabbit anti-Human, Mouse, Rat, Polyclonal, Rockland™
Manufacturer: Rockland Immunochemicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-1739-Each-of-1 | In Stock | ₹ 52,930.08 |
50-199-1739 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,930.08
GST (18%): ₹ 9,527.414
Total Price: ₹ 62,457.494
Antigen
Phospho-Mdm2 (Ser185)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
MDM2
Gene Alias
1700007J15Rik; AA415488; ACTFS; double minute 2; Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; HGNC:6973; human homolog of; Mdm2; Mdm-2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog; MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 E3 ubiquitin protein ligase; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed mouse 3T3 cell double minute 2
Host Species
Rabbit
Regulatory Status
RUO
Gene ID (Entrez)
17246, 314856, 4193
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Applications
ELISA, Immunoprecipitation, Western Blot
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Accession No.
P23804, Q00987
Gene Symbols
MDM2
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2 protein at the pS185 residue
- Reactivity occurs against Mouse MDM2 pS185 protein and the antibody is specific for the phosphorylated form of the protein
- Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot
- A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources
- MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53
- MDM2 is located on chromosome 12 on the q arm
- Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53
- Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53
- MDM2 has also been shown to negatively regulate p53 function
- MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function
- Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity
- Further, MDM2 plays important roles in apoptosis and cell cycle
- MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer
- In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5
- More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.