IgD Monoclonal Antibody (IA6-2), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predomit (GARP, also known as LRRC32 or Garpin)
• GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface
• Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas
• Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells
• The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta
• Applications Reported: This G14D9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This G14D9 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.