CD223 (LAG-3) Monoclonal Antibody (3DS223H), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3)
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells)
• This 3DS223H antibody will recognize a formaldehyde-fixed epitope
• Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).