5350421
MilliporeSigma™ anti-MHC class II (I-A/I-E), APC, Clone: M5/114.15.2,
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
MHC class II (I-A/I-E)
Applications
Flow Cytometry
Conjugate
APC
Host Species
Rat
Research Discipline
Inflammation, Immunology
Formulation
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols
CD74, Ii
Isotype
IgG2b κ
Purification Method
Purified by size-exclusion chromatography
Clone
M5/114.15.2
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Accession No.
P04441
Immunogen
Proprietary immunogen
Primary or Secondary
Primary
Content And Storage
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
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Description
- Specifically detects MHC class II (I-A/I-E) Clone: M5/114.15.2 in Mouse samples, and it is validated for Flow Cytometry The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype
- MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells
- The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described
- Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.