IgD Monoclonal Antibody (IA6-2), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD)
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding
• Applications Reported: This IA6-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This IA6-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.