62999842
IgM Monoclonal Antibody (SA-DA4), Super Bright™ 436, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 62999842-Each-of-1 | In Stock | ₹ 31,773.00 |
62999842 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,773.00
GST (18%): ₹ 5,719.14
Total Price: ₹ 37,492.14
Antigen
IgM
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P01871
Gene Symbols
IGHM
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3507
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
SA-DA4
Conjugate
Super Bright 436
Gene
IGHM
Gene Alias
AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM
- It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
- IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
- Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific
- This variation can be attributed to both donor variability and the anticoagulant used during blood draw
- Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody
- Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
- We recommend using a 450/50 bandpass filter, or equivalent
- Please make sure that your instrument is capable of detecting this fluorochrome
- IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.