CD105 (Endoglin) Monoclonal Antibody (SN6), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin
• CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of U937 cell line
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.