IgD Monoclonal Antibody (11-26c (11-26)), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells
• The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression
• Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression
• Applications Reported: This 8A7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 8A7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.