7068444
Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Phospho-4EBP1 (Thr36, Thr45)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
4EBP1; 4E-BP1; AA959816; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Gene Symbols
EIF4EBP1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
EIF4EBP1
Clone
V3NTY24
Applications
Flow Cytometry
Conjugate
PE
Host Species
Mouse
Target Species
Human, Mouse
Gene Accession No.
Q13541, Q60876
Gene ID (Entrez)
13685, 1978
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46
- 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3
- In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation
- Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation
- Studies have identified several kinases that phosphorylate 4E-BP1
- For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111
- Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin
- Applications Reported: This V3NTY24 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This V3NTY24 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells
- This can be used at 5 μL (0.06 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- This gene encodes one member of a family of translation repressor proteins
- The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs
- Interaction of this protein with eIF4E inhibits complex assembly and represses translation
- This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.