Blimp-1 Monoclonal Antibody (5E7), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Blimp-1
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
B lymphocyte-induced maturation protein 1; b2b1765Clo; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; Blimp1; BLIMP-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR/SET domain 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; Prdm1; RP1-134E15.1; ZNFPR1A1
Gene Symbols
PRDM1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
PRDM1
Clone
5E7
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q60636
Gene ID (Entrez)
12142
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1
- Blimp-1 is a transcriptional repressor with five zinc finger domains
- Blimp-1 is expressed by terminally differentiating B and T cells
- Blimp-1 is also expressed by some dendritic cells, and some NK cells
- This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation
- While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation
- Blimp-1 promotes effector molecule secretion while suppressing proliferation
- Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation
- Applications Reported: The 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: The 5E7 antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus
- It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages
- Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells
- It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.