7105361

ICAM-3/CD50 Antibody (SPM505), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

ICAM-3/CD50

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P32942

Gene ID (Entrez)

3385

Immunogen

Stimulated human leukocytes

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Clone

SPM505

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cellular Markers, Immunology

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3

Gene Symbols

ICAM3

Isotype

IgG2b κ

Purification Method

Protein A purified

Test Specificity

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

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Description

  • ICAM-3/CD50 Monoclonal specifically detects ICAM-3/CD50 in Human samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.