Aquaporin 4, Rabbit anti-Rat, Mouse, Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Aquaporin 4
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Rat, Mouse, Human
Gene Alias
AQP-4;Mercurial-insensitive water channel;MIWC
Gene Symbols
Aqp4
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution
Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
Gene ID (Entrez)
NP_001257487.1
Immunogen
GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.
Related Products
Description
- Anti-Aquaporin 4, Cat
- No
- ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Aquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel, MIWC) is encoded by the Aqp4 gene (Gene ID: 25293) in rat
- Aquaporin-4 is a multi-pass membrane homotetrameric protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system
- Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323)
- Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue
- Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%
- Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing
- Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis
- Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4
- (Ref.: Hinson, SR et al
- (2017)
- Proc
- Natl
- Acad
- Sci
- USA 114(21); 5491-5496).