carboxypeptidase M, Mouse anti-Rat, Clone: 7F9, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
carboxypeptidase M
Dilution
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Rat
Gene Alias
CPM;CP-M
Gene Symbols
Cpm
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone
7F9
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Signaling
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001101568.1
Immunogen
Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Related Products
Description
- Anti-carboxypeptidase M, clone 7F9, Cat
- No
- MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting
- Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat
- Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH
- Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form
- The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates
- It is shown to be expressed in the lung and placenta at high levels
- Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung
- Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages
- Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages
- Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1
- (Ref.: Fujiwara, N., et al
- (2007), Respirology 12(1); 54-62; Tsakiris, I., et al
- (2012)
- Lab
- Invest
- 92(3); 345-361).