Lymphotoxin-alpha/TNF-beta, Mouse anti-Human, Clone: 3F12.2D3, Novus Biologicals™

Lymphotoxin-alpha/TNF-beta

1 mg/mL

Flow Cytometry, ELISA, Neutralization

Unconjugated

Mouse

Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity

PBS

4049

3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.

Primary

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

3F12.2D3

Flow Cytometry, ELISA, Surface Plasmon Resonance, Neutralization

Monoclonal

Purified

RUO

Human

LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1

LTA

IgG2b κ

Protein A purified

3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.