Fas/TNFRSF6/CD95, Human anti-Human, Clone: R-125224, Novus Biologicals™

Fas/TNFRSF6/CD95

1 mg/mL

Flow Cytometry, ELISA, Functional Assay

Unconjugated

Human

Human

Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6

FAS

IgG1 κ

Protein A purified

R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.

R-125224

Flow Cytometry, ELISA, Functional

Monoclonal

Purified

RUO

PBS

355

R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.

Primary

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.