SaCas9 Recombinant Mouse Monoclonal Antibody, AbFlex™, Active Motif™
Mouse Recombinant Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SaCas9
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human
Gene Accession No.
P55211
Gene ID (Entrez)
842
Immunogen
This antibody was raised against a recombinant protein fragment containing amino acids 1-462 of Staphylococcus aureus Cas9.
Primary or Secondary
Primary
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Gene
CASP9
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
140mM HEPES, 32mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Gene Alias
APAF3; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP9; CASP-9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; CRISPR-Cas9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; OTTHUMP00000002324; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3
Gene Symbols
CASP9
Isotype
IgG2a
Purification Method
Protein A
Product Type
Antibody
Related Products
Description
- Cas9 is a RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering
- Staphylococcus aureus derived Cas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity
- SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 (Cas9 derived from S
- pyogenes) with an ideal spacer lengths of 24 to 20 nucleotides
- In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM
- This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.