CD271 Mouse anti-Human, BB515, Clone: C40-1457, BD

Description

• The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR)
• CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily
• CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves
• It is also expressed by some epithelial, mesenchymal and lymphoid tissues
• NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system
• NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system
• NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]
• High affinity binding of NGF requires that both receptor molecules be expressed
• NGFR is expressed on human and rat lymphocytes
• A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271
• It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation
• NGF may also function as a B-cell growth factor
• The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.