Hepatitis C Virus Core Antigen Monoclonal Antibody (C7-50), Invitrogen™

Description

• MA1-080 detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines
• MA1-080 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures
• By Western blot, this antibody detects a single ∽21 kDa protein representing HCV core protein in various transfected cell lines
• Immunofluorescence staining of HCV core protein in transfected chimp hepatocytes yields a staining pattern consistent with cytoplasmic and vesicular staining
• The MA1-080 immunogen is purified HCV core-GST fusion protein (genotype 1b)
• This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein
• This sequence is conserved among different HCV strains
• HCV is a positive, single-stranded RNA virus in the Flaviviridae family
• The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids
• The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication
• Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.