APE1 Monoclonal Antibody (13B 8E5C2), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA1440-Each-of-1 | In Stock | ₹ 50,285.00 |
MA1440 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,285.00
GST (18%): ₹ 9,051.30
Total Price: ₹ 59,336.30
Antigen
APE1
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 0.05% sodium azide
Gene Accession No.
P27695, P28352, P43138
Gene Symbols
APEX1
Immunogen
Full-length recombinant human APE protein.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Canine, Human, Mouse, Non-human Primate, Rat
Product Type
Antibody
Isotype
IgG2a
Applications
ChIP Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immunoprecipitation, Western Blot
Clone
13B 8E5C2
Conjugate
Unconjugated
Gene
APEX1
Gene Alias
AP endonuclease 1; AP endonuclease class I; AP lyase; Ape; APE1; APEN; Apex; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apex1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endodeoxyribonuclease 1; apurinic/apyrimidinic endonuclease; apurinic/apyrimidinic endonuclease 1; Apurinic-apyrimidinic endonuclease 1; APX; BAP 1; BAP1; deoxyribonuclease (apurinic or apyrimidinic); DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase, mitochondrial; HAP1; protein REF-1; redox factor 1; redox factor-1; REF1; REF-1
Host Species
Mouse
Purification Method
purified
Regulatory Status
RUO
Gene ID (Entrez)
11792, 328, 482558, 79116
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non human primate, canine, mouse and rat samples
- MA1-440 has been successfully used in Western blot, immunohistochemical, flow cytometry and immunoprecipitation procedures
- By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate
- Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining
- The MA1-440 immunogen is full-length, recombinant human APE protein
- Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions
- APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis
- The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases
- APE is differentially expressed during development and in different tissues
- This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus
- Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II
- Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue
- Decreases in APE expression have been associated with the induction of cellular apoptosis.