MilliporeSigma™ anti-alpha-Tubulin Clone: YL 1/2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MAB1864IMI-Each-of-1 | In Stock | ₹ 44,799.04 |
MAB1864IMI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,799.04
GST (18%): ₹ 8,063.827
Total Price: ₹ 52,862.867
Antigen
alpha-Tubulin
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
Q71U36
Host Species
Rat
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Reacts with alpha-tubulin with phenylalanine (yeast) or tyrosine (mammalian and chicken) at the C-terminus (PMID 6204858). Does not react toward posttranslationally modified alpha-tubulin lacking the C-terminus Phe or Tyr.
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG2a κ
Applications
Immunoassay, ELISA, Immunocytochemistry, Immunohistochemistry, Radioimmune Assays (RIA), Western Blot
Clone
YL 1/2
Formulation
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
TUBA1A, TUBA3
Immunogen
Purified yeast tubulin
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_013625
Target Species
Bovine, Chicken, Human, Mouse, Porcine, Rat, Yeast
Form
Purified
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Description
- Specifically detects alpha-Tubulin Clone: YL 1/2 in Bovine, Chicken, Human, Mouse, Porcine, Rat, Yeast samples, and it is validated for Electron Microscopy, ELISA, Immunocytochemistry, Immunohistochemistry, Radioimmunoassay, Western Blotting Tubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast
- Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape
- Tubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast
- Microtubules are dynamic protein filaments involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape
- Microtubules are assembled from α- and β-tubulin subunits, both of which are subjected to posttranslational modifications, including the removal of the C-terminus aromatic amino acid from α-tubulin (tyrosine in mammals and phenylalanine in yeast) by carboxypeptidase actifvity
- Because the C-terminus tyrosine/phenylalanine is preceded by glutamate (E) residues, the detyrosinated/dephenylalaninated α-tubulin is also referred to as Glu-tubulin
- While tyrosine can be reattached to the C-terminus of the Glu-tubulin by tubulin-tyrosine ligase in mammals, phenylalanine is not added posttranslationally to the Glu-tubulin in yeast despite the presence of the yeast YBR094w gene that exhibits significant homology to tubulin-tyrosine ligase in other organisms.