MilliporeSigma™ anti-Laminin-5 Alexa Fluor 647, Clone: D4B5,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MAB19562AF6-Each-of-1 | In Stock | ₹ 50,816.33 |
MAB19562AF6 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,816.33
GST (18%): ₹ 9,146.939
Total Price: ₹ 59,963.269
Antigen
Laminin-5
Classification
Monoclonal
Conjugate
Alexa Fluor 647
Gene Accession No.
Q13753-1
Host Species
Mouse
Purification Method
Protein A purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody recognizes the 150 (precursor form) and 105kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG1
Applications
Immunocytochemistry
Clone
D4B5
Formulation
Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Gene Symbols
LAMC2, LAMB2T, LAMNB2
Immunogen
Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_005553
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Specifically detects Laminin-5 Clone: D4B5 in Human, Mouse samples, and it is validated for Immunocytochemistry Laminin subunit gamma-2 (UniProt Q13753; also known as CSF 140kDa subunit, Cell-scattering factor 140kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140kDa subunit, Laminin B2t chain, Lamin-5 subunit gamma, Large adhesive scatter factor 140kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as B2T, LAMB2T, LAMNB2) gene (Gene ID 3918) in human
- Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta and gamma heterotrimeric chains
- Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms
- Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes
- Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease
- LN5 may stabilize the epidermal/dermal junction of skin through interaction with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes
- Comparing to fibronectin, collagen, and vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent
- Laminin-5 attracted attention when it was detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, T., et al
- (2002)
- Am
- J
- Pathol
- 160(3):112911-41; Lohi, J
- (2001)
- Int
- J
- Cancer
- 94(6):763-767).