MilliporeSigma™ anti-CEP Clone: 7E12H12,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABC530MI-Each-of-1 | In Stock | ₹ 42,968.31 |
MABC530MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,968.31
GST (18%): ₹ 7,734.296
Total Price: ₹ 50,702.606
Antigen
CEP
Classification
Monoclonal
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Purified by ion-exchange chromatography
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone
7E12H12
Formulation
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Immunogen
Human colonic protein extract.
Quantity
100 μL
Research Discipline
Apoptosis, Cancer
Test Specificity
Clone 7E12H12 (mAb DAS-1) specifically reacts with Colon Epithelial Protein (CEP), but not human tropomyosins isoforms (hTM1-5). CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, HUVEC, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgM κ
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Description
- Specifically detects CEP Clone: 7E12H12 in Human samples, and it is validated for Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting The Monoclonal (mAb) Das-1 was originally desiganted as clone 7E12H12 and was thought to target a 40kDa colon-specific tropomyosin (TM) isoform or a TM-related protein
- It was later found that this clone specifically immunoprecipitated a high molecular weight chaperone protein that targets tropomyosin 5 (TM5) in human colon epithelial cells and subsequently named Colon Epithelial Protein (CEP)
- The Das-1 target is a glycoprotein with variable apparent sizes depending on the level of glycosylation
- The target glycoprotein immunoprecipitated from the plasma membrane of DLD-1 colon cancer cells appears with an apparent molecular size of 185kDa, while the target isolated from LS-180 colon cancer cells migrates with a size range extends to greater than 200kDa
- Das-1/CEP immunoreactivity is reported to be associated with Barrett's metaplasia, Barrett's adenocarcinoma, as well as with gastric adenocarcinomas and carcinoma-associated intestinal metaplasia, but no reactivity has been found in normal esophageal, gastric or small intestinal mucosa
- When tested by ELISA and imunoblotting against recombinant human tropomyosins isoforms 1-5, none of five hTM isoforms reacted with mAB DAS-1
- As determined by immunofluorescence and FACS analyses, CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29
- CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).