MilliporeSigma™ APE/Ref-1, Mouse, Unlabeled, Clone: 13B8E5C2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1068MI-Each-of-1 | In Stock | ₹ 38,958.86 |
MABE1068MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,958.86
GST (18%): ₹ 7,012.595
Total Price: ₹ 45,971.455
Antigen
APE/Ref-1
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P27695
Immunogen
Recombinant full-length human APE/Ref-1.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_001632.2
Target Species
Human
Form
Purified
Applications
Immunohistochemistry (Paraffin), Western Blot
Clone
13B8E5C2
Conjugate
Unconjugated
Gene
APEX1, APE, APE1, APEX, APX, HAP1, REF1
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 13B8E5C2 detected both cytosolic and nuclear APE/Ref-1 by Immunocytochemistry, Immunohistochemistry, and Western blotting (Moore, D.H., et al. (2000). Clin. Cancer Res. 6(2):602-609).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a κ
Related Products
Description
- DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18; UniProt P27695; also known as AP endonuclease 1, APE-1, APEN, APEX nuclease, Apurinic-apyrimidinic endonuclease 1, Redox factor-1, REF-1) is encoded by the APEX1 (also known as APE, APE1, APEX, APX, HAP1, REF1) gene (Gene ID 328) in human
- APE/Ref-1 mediates the cleavage of an apurinic/apyrimidinic (AP) site in DNA generated by DNA glycosylases during base excision repair (BER) in both nucleus and mitochondria
- APE/Ref-1 also functions as a redox factor (ref) responsible for maintaining several transcription factors (TFs) in an active reduced state for DNA binding, including AP-1, NF-κB, HIF-1α, CREB, TP53
- Elevated levels of APE1 are associated with resistance to chemotherapyand poor prognosis in various cancers
- APE/Ref-1 is synthesized as a 318-a.a
- protein whose initiation methionine (Met1) is removed posttranslationally to yield the 317-a.a
- form with a nuclear localization sequence (NLS; a.a
- 8-13) and a nuclear export signal (NES; a.a
- 64-80) sequence to allow its shuttle between the cytoplasm and nucleus
- The 317-a.a
- form can be further processed by a mitochondria-associated N-terminal peptidase into a mitochrondrial form (mtAPE; a.a
- 32-318) that lacks NLS and contains only the NES and the mitochondrial targeting sequence (MTS; a.a
- 289-318).