MilliporeSigma™ DNA-RNA Hybrid, Mouse, Unlabeled, Clone: S9.6,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1095MI-Each-of-1 | In Stock | ₹ 47,575.84 |
MABE1095MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,575.84
GST (18%): ₹ 8,563.651
Total Price: ₹ 56,139.491
Antigen
DNA-RNA Hybrid
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen
DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a κ
Applications
ChIP Assay, Dot Blot, Immunocytochemistry, Immunoprecipitation
Clone
S9.6
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
All species
Form
Purified
Related Products
Description
- DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity
- They are non-canonical nucleic acid structures with transcriptional regulatory functions
- Their presence is reported to predispose a locus to chromosomal breakage
- A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand
- They are shown to be resistant to the activity of DNA methyltransferases
- The formation of DNA:RNA hybrids has been associated with a number of neurological diseases
- Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.