MABE1167MI
MilliporeSigma™ SPO11, Mouse, Unlabeled, Clone: 180,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1167MI-Each-of-1 | In Stock | ₹ 44,393.20 |
MABE1167MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,393.20
GST (18%): ₹ 7,990.776
Total Price: ₹ 52,383.976
Antigen
SPO11
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q9WTK8
Immunogen
Recombinant mouse SPO11 with N-terminal S-tag and C-terminal His-tag.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_036176.1
Target Species
Mouse
Form
Purified
Applications
Immunoprecipitation, Western Blot
Clone
180
Conjugate
Unconjugated
Gene
Spo11
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 180 detected SPO11alpha and SPO11beta in wild-type, but not Spo11-knockout mouse testes lysates by IP-Western blotting.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a κ
Related Products
Description
- Meiotic recombination protein SPO11 (UniProt Q9WTK8) is encoded by the Spo11 gene (Gene ID 26972) in murine species
- SPO11 is a widely conserved protein that is involved in meiotic recombination and mediates DNA cleavage that forms the double-strand breaks that initiate meiotic recombination
- Active site of SPO11 contains a tyrosine, which ligates and dissociates with DNA to promote break formation
- One SPO11 protein is involved per strand of DNA, hence, two SPO11 proteins are involved in each double stranded break event
- High levels of SPO11 are found only in the testis where expression is restricted primarily to meiotic germ cells and it is not expressed in spermatogonia
- Very low levels of SPO11 have been reported in thymus, brain, and oocytes of embryonic ovary, but it is not detected in adult ovary
- Mouse and human SPO11 exhibit about 82% sequence homology
- Ref.: Keeney, S., et al
- (1997)
- Cell 375-384.