MABE974MI
MilliporeSigma™ Mouse anti-Ser/Thr-protein kinase Nek2, Clone: 5D2.1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE974MI-Each-of-1 | In Stock | ₹ 40,292.08 |
MABE974MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,292.08
GST (18%): ₹ 7,252.574
Total Price: ₹ 47,544.654
Antigen
Ser/Thr-protein kinase Nek2
Classification
Monoclonal
Formulation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
NEK2; NEK2A; NLK1
Immunogen
GST-tagged recombinant protein corresponding to the N-terminal of human Ser/Thr-protein kinase Nek2.
Quantity
100 μg
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_002488
Content And Storage
Stable for one year at 2°C to 8°C from date of receipt.
Isotype
IgG2b κ
Applications
Immunocytochemistry, Western Blot
Clone
5D2.1
Gene Accession No.
P51955
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Specifically detects Ser/Thr-protein kinase Nek2 clone: 5D2.1 in Human, Mouse samples, and it is validated for Immunocytochemistry, Western Blotting Serine/threonine-protein kinase Nek2 (EC 2.7.11.1; UniProt P51955; also known as HSPK 21, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, Protein phosphatase 1 regulatory subunit 111) is encoded by the NEK2 (also known as NEK2A, NLK1, PPP1R111, RP67) gene (Gene ID 4751) in human
- Nek2 is a member of the never in mitosis A- (NIMA-) related family of serine/threonine kinases
- Nek2 is involved in cell cycle regulation, gene expression, and the maintenance of centrosomal structure and function
- Cellular Nek2 levels are tightly regulated throughout the cell cycle with noncentrosomal Nek2 expression peaks from S to G2 phase, while cytoplasmic Nek2 is highest in G1, and nuclear Nek2 is highest during S and G2 phase when it is needed for centrosomal function regulation
- During interphase, centrosomes are held together by a proteinaceous linker that connects the the mother and daughter centriole
- This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction
- The mammalian sterile 20-like kinase 2 (Mst2) and the scaffold protein Salvador (hSav1) are shown to directly interact with Nek2A and regulate its ability to localize to centrosomes, where Nek2A mediating linker dissociation by phosphorylating the centrosomal linker proteins C-Nap1 and rootletin
- In addition, Nek2 is also reported to regulate gene expression by phosphorylating the splicing factor SRSF1
- Nek2 expression is found upregulated in a wide variety of neoplasms including diffuse large B cell lymphoma and multiple myeloma.