PA3514
Glucocorticoid Receptor beta Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PA3514-Each-of-1 | In Stock | ₹ 46,413.50 |
PA3514 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,413.50
GST (18%): ₹ 8,354.43
Total Price: ₹ 54,767.93
Antigen
Glucocorticoid Receptor beta
Classification
Polyclonal
Conjugate
Unconjugated
Gene
NR3C1
Gene Alias
GCCR; GCR; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; GR; GR Beta; GRL; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Host Species
Rabbit
Quantity
50 μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Concentration
Conc. Not Determined
Formulation
whole serum diluted in PBS with 0.05% sodium azide
Gene Accession No.
P04150
Gene Symbols
NR3C1
Immunogen
Synthetic Peptide: N(728) V M W L K P E S T S H T L I(742) C
Regulatory Status
RUO
Gene ID (Entrez)
2908
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- PA3-514 detects glucocorticoid receptor (GR) beta from human cells
- PA3-514 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures
- By Western blot, this antibody detects a ∽90 kDa protein representing GR beta from HeLa cell lysate
- Immunocytochemical staining of GR beta in HeLa cells with PA3-514 results in nuclear staining
- PA3-514 immunizing peptide corresponds to amino acid residues 728-742 from human GR beta
- This peptide (Cat
- # PEP-222) is available for use in neutralization and control experiments
- Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses
- These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA
- All normal mammalian tissues examined to date have been shown to contain GR
- The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene
- Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively
- They are identical up to residue 727, after which they diverge
- After ligand binding, the 94 kDa GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression
- In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines
- Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.