Glucocorticoid Receptor Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PA1512-Each-of-1 | In Stock | ₹ 50,685.50 |
PA1512 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,685.50
GST (18%): ₹ 9,123.39
Total Price: ₹ 59,808.89
Antigen
Glucocorticoid Receptor
Classification
Polyclonal
Conjugate
Unconjugated
Gene
Nr3c1
Gene Alias
cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
14815, 24413, 2908
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Lyophilized
Applications
ChIP Assay, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
Concentration
1 mg/mL
Formulation
antibody with no preservative
Gene Accession No.
P04150, P06536, P06537
Gene Symbols
Nr3c1
Immunogen
Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.
Quantity
50 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- PA1-512 detects glucocorticoid receptor (GR) from human, mouse and rat tissues
- PA1-512 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, ChIP and gel shift procedures
- By Western blot, this antibody detects an ∽97 kDa protein representing GR as well as two other unidentified proteins at ∽135 to ∽145 kDa from rat liver extract
- These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate
- Immunohistochemical staining of GR in rat hippocampus using PA1-512 results in staining primarily in the nucleus
- PA1-512 does not disrupt receptor-DNA interactions
- The PA1-512 immunogen is a synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR
- The PA1-512 immunizing peptide (Cat
- # PEP-002) is available for use in for neutralization and control experiments
- Reconstitute in 50 μL PBS to create a stock of 1 mg/mL
- Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors
- Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins
- The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus
- Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart
- Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined
- Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance
- Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation
- GR binds to known steroids such as dexamethasone with nanomolar affinity