Vimentin Monoclonal Antibody (SP20), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA516409-Each-of-1 | In Stock | ₹ 56,426.00 |
PIMA516409 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,426.00
GST (18%): ₹ 10,156.68
Total Price: ₹ 66,582.68
Antigen
Vimentin
Classification
Monoclonal
Concentration
0.006 mg/mL
Formulation
98.9% PBS with 1% BSA and 0.1% sodium azide; pH 7.20
Gene Accession No.
P08670
Gene Symbols
Vim
Immunogen
Recombinant human vimentin protein.
Quantity
500 μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone
SP20
Conjugate
Unconjugated
Gene
Vim
Gene Alias
cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
7431
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
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Description
- Predicted to react with Rat, Hamster, Cow, and Xenopus laevis based on sequence homology
- This antibody is not suitable for mouse tissues
- Recommended Postive Controls - WB: U-2 OS, Hu tonsil and HeLa cell lysates
- Flow: HeLa cells
- ICC/IF: HAP1-VIM cells, human limbal epithelial cells
- IHC-P: Human breast cancer and melanoma tissue
- IHC-Fr: Colorectal cancer tissue
- Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin
- It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane
- Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development
- During the development process, vimentin is exchanged for new, tissue-specific IFPs
- Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets
- Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event
- This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.