iNOS Monoclonal Antibody (SP126), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA516422-Each-of-1 | In Stock | ₹ 61,944.00 |
PIMA516422 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 61,944.00
GST (18%): ₹ 11,149.92
Total Price: ₹ 73,093.92
Antigen
iNOS
Classification
Monoclonal
Concentration
Conc. Not Determined
Formulation
PBS with 1% BSA and 0.1% sodium azide; pH 7.6
Gene Accession No.
P29477, P35228
Gene Symbols
Nos2
Immunogen
Synthetic peptide near the C-terminus of mouse iNOS protein.
Quantity
500 μL
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
Applications
Flow Cytometry, Immunohistochemistry (Paraffin)
Clone
SP126
Conjugate
Unconjugated
Gene
Nos2
Gene Alias
Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2
Host Species
Rabbit
Purification Method
Protein A/G
Regulatory Status
RUO
Gene ID (Entrez)
18126, 4843
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min
- Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature
- A suggested positive control is lung tissue
- iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells
- Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO
- NO production is mediated by members of the nitric oxide synthase (NOS) family
- iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines
- NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO
- Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned
- All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains
- iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells
- Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels
- Protein kinase C-stimulating agents exhibit the same effect on iNOS activity
- After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time
- Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17
- Diseases associated with iNOS dysfunction include achalasia and impotence.