ROCK1 Recombinant Rabbit Monoclonal Antibody (ST05-19), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA532167-Each-of-1 | In Stock | ₹ 46,413.50 |
PIMA532167 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,413.50
GST (18%): ₹ 8,354.43
Total Price: ₹ 54,767.93
Antigen
ROCK1
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
Gene Accession No.
P70335, Q13464, Q63644
Gene Symbols
Rock1
Immunogen
Synthetic peptide within Human ROCK1 aa aa 1104-1149
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunohistochemistry, Western Blot
Clone
ST05-19
Conjugate
Unconjugated
Gene
Rock1
Gene Alias
1110055K06Rik, Ac2-154, Liver regeneration-related protein LRRG199, MGC131603, MGC43611, p150 RhoA-binding kinase ROK beta, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho associated coiled-coil containing protein kinase 1, Rho kinase, Rho-associated coiled-coil containing protein kinase 1, Rho-associated coiled-coil forming kinase 1, Rho-associated kinase beta, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK1, ROCK-I
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
19877, 6093, 81762
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration
- The Rho/ROCK pathway is involved in the progression of various human cancers
- ROCK1 has been found to be a new Caspase-3 substrate
- ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition
- The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation
- ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region
- They are joined by a variable region that contains the Rho-binding domain
- During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity
- The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.