IL5RA (soluble) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA124792-Each-of-1 | In Stock | ₹ 46,591.50 |
PIPA124792 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,591.50
GST (18%): ₹ 8,386.47
Total Price: ₹ 54,977.97
Antigen
IL5RA (soluble)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
IL5RA
Gene Alias
CD125; CD125 antigen; CDw125; HSIL5R3; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; Il5r; IL-5R subunit alpha; Il5ra; IL-5RA; IL-5R-alpha; interleukin 5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor subunit alpha
Host Species
Goat
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3568
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Applications
Flow Cytometry, Inhibition Assays, Neutralization, Western Blot
Concentration
0.1 mg/mL
Formulation
PBS with 5% trehelose and no preservative; pH 7.4
Gene Accession No.
Q01344
Gene Symbols
IL5RA
Immunogen
Purified, recombinant human interleukin 5 soluble receptor alpha (IL5RA) expressed in insect Sf 21 cells.
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Recommended positive controls: human blood-derived granulocytes
- The Neutralization Dose50 (ND50) for anti-human IL5RA is approximately 1-3 μg/mL in the presence of 0.5 ng/mL of recombinant human IL-5 using the human TF-1 cell line
- The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cell surface IL5RA mediated IL5 response on a responsive cell line, at a specific IL5 concentration
- The exact concentration of antibody required to neutralize the human cell surface IL5RA mediated bioactivity is dependent on the IL5 concentration as well as on the number of IL5RA present on the cell surface (a function of the cell type and culture conditions)
- Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation
- IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor
- The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction
- Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.