Phospho-IRS1 (Ser307) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143361-Each-of-1 | In Stock | ₹ 39,427.00 |
PIPA5143361 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 39,427.00
GST (18%): ₹ 7,096.86
Total Price: ₹ 46,523.86
Antigen
Phospho-IRS1 (Ser307)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
IRS1
Gene Alias
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16367, 25467, 3667
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Applications
Immunohistochemistry (Paraffin), Western Blot
Concentration
1.51 mg/mL
Formulation
PBS with 1% BSA, 20% glycerol and 0.025% Proclin 300; pH 7
Gene Accession No.
P35568, P35569, P35570
Gene Symbols
IRS1
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser307 of human IRS1. The exact sequence is proprietary.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Store as concentrated solution
- Centrifuge briefly prior to opening vial
- Positive control: C2C12 (100 nM Insulin treatment for 30 min)
- IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
- IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
- An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
- Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
- Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
- IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.