Phospho-eNOS/nNOS (Tyr657, Tyr895) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143729-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143729 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-eNOS/nNOS (Tyr657, Tyr895)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
NOS3
Gene Alias
endothelial Nitric Oxide Synthase; eNOS; NOS-III
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
18127, 24600, 4846
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Immunocytochemistry, Western Blot
Concentration
∽1.0 mg/mL
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P29474, P70313, Q62600
Gene Symbols
NOS3
Immunogen
Phospho-eNOS (Tyr-657) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 657 in human eNOS. This sequence is conserved in mouse (Tyr-656) and rat (Tyr-656) eNOS, and is identical to the conserved site in nNOS (Tyr-895). The site is also well conserved in iNOS (Tyr-631).
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- The antibody detects a 140 kDa* band on SDS-PAGE immunoblots of human umbilical vein endothelial cells treated with pervanadate, and this reactivity is not observed after akaline phosphatase treatment
- Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species
- Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme)
- Several distinct NOS isoforms are produced from three distinct genes
- The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells
- Regulation of eNOS activity occurs through phosphorylation at multiple sites
- Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation.