Phospho-N-cadherin/E-cadherin (Tyr820, Tyr835, Tyr860) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143660-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143660 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-N-cadherin/E-cadherin (Tyr820, Tyr835, Tyr860)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
CDH2
Gene Alias
Cadherin-2; CD325
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
1000, 12558, 83501
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Immunocytochemistry, Western Blot
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P15116, P19022, Q9Z1Y3
Gene Symbols
CDH2
Immunogen
Phospho-N-Cadherin (Tyr-860) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 860 in human N-cadherin. This sequence is conserved in human E-cadherin (Tyr-835), P-cadherin (Tyr-782), R-cadherin (Tyr-870), and has significant homology to VE-Cadherin (Tyr-733).
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide
- In western blots, the antibody detects a 130 kDa* band corresponding to N-cadherin (Tyr-860) in human endothelial cells treated with pervanadate and detects a 120 kDa band corresponding to E-cadherin (Tyr-835) in human A431 cells treated with pervanadate
- Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation
- Cadherins cluster to form foci of homophilic binding units
- A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins
- This region induces clustering and also binds to the protein p120 catenin
- The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton
- Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin
- N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886
- Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin
- Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.