Phospho-DOK1/DOK2 (Tyr362, Tyr337) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143681-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143681 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-DOK1/DOK2 (Tyr362, Tyr337)
Classification
Polyclonal
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P97465, Q4QQV2, Q99704
Gene Symbols
DOK1
Immunogen
Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Applications
Western Blot
Clone
WB, E
Conjugate
Unconjugated
Gene
DOK1
Gene Alias
p62DOK
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
13448, 1796, 312477
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Related Products
Description
- This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide
- The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment
- Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified
- Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues
- When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins
- Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk
- Several tyrosine phosphorylation sites have been identified for Dok1
- One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin
- Alternatively, Dok1 activity is also regulated by serine phosphorylation
- IkappaB Kinase beta phosphorylates several serine sites including Ser-450 in vitro, and TNFalpha, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo
- Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.