Phospho-Profilin 1 (Ser138) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143752-Each-of-1 | In Stock | ₹ 47,570.50 |
PIPA5143752 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,570.50
GST (18%): ₹ 8,562.69
Total Price: ₹ 56,133.19
Antigen
Phospho-Profilin 1 (Ser138)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
Pfn1
Gene Alias
actin binding protein; ALS18; Epididymis tissue protein Li 184a; Pfn; Pfn1; profilin 1; profilin I; Profilin-1
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
18643, 5216, 64303
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Western Blot
Concentration
∽0.5 mg/mL
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P07737, P62962, P62963
Gene Symbols
Pfn1
Immunogen
Phospho-Profilin (Ser138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser138 in human Profilin-1. This sequence is conserved in rat and mouse Profilin-1. The serine site has some homology to the conserved site (Ser138) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- This antibody was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier)
- The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human Jurkat and A431 cells, as well as rat PC12 cells
- This reactivity is decreased by lambda phosphatase treatment, and is blocked by phospho-profilin (Ser-138) blocking peptide
- Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics
- There are two mammalian profilins with similar biochemical properties but different expression patterns
- Profilin I appears to be highly expressed in most tissues except for skeletal muscle
- Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney
- Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.