Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143756-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143756 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
BRAF
Gene Alias
BRAF1; CRaf; Raf1; RAFB1
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
109880, 110157, 114486, 24703, 5894, 673
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Western Blot
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P04049, P11345, P15056, P28028, Q99N57
Gene Symbols
BRAF, RAF1
Immunogen
Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
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Description
- This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide
- The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A
- The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells
- The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation
- The Raf kinase family includes A-Raf, B-Raf, and C-Raf
- Each family member has three highly conserved regions (CR1-3)
- The N-terminal CR1 contains the Ras-GTP-binding domain
- The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins
- The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment
- Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites
- In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain
- Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation
- Ser-579 is required for growth factor activation and kinase activity
- Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.