PIPA532516

Nestin Polyclonal Antibody, Invitrogen™

Manufacturer: Thermo Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PIPA532516-Each-of-1 In Stock ₹ 45,390.00

PIPA532516 - Each of 1

₹ 45,390.00

In Stock

Quantity

1

Base Price: ₹ 45,390.00

GST (18%): ₹ 8,170.20

Total Price: ₹ 53,560.20

Antigen

Nestin

Classification

Polyclonal

Gene

NES

Gene Alias

NES, FLJ21841, Nbla00170

Host Species

Rabbit

Purification Method

Antigen affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

10763

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form

Liquid

Applications

Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Gene Accession No.

P48681

Gene Symbols

NES

Immunogen

Synthetic peptide derived from C-terminus of human nestin protein.

Quantity

500 μL

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG

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Description

  • Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min
  • Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature
  • A suggested positive control is liver hepatoma
  • Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
  • Nestin has been widely used as a predomit marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
  • Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
  • Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
  • Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.